Proteasome 20S beta 3 Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9357R-Cy5 |
| Product Name | Proteasome 20S beta 3 Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Proteasome 20S beta 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5684 |
| Synonyms | 20S Proteasome _3; C10 II; HC10 II; proteasome prosome, macropain subunit, beta type 3; Proteasome beta 3 subunit; Proteasome chain 13; proteasome component C10 II; Proteasome theta chain; PSMB3. |
| Background | The proteasome is a multicatalytic proteinase complex with a highly ordered ring shaped 20S core structure. The core structure is composed of 4 rings of 28 non identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin dependent process in a non lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is Proteasome 20S beta 3 Antibody, Cy5 Conjugated (bs-9357R-Cy5) validated for, and at what dilution?
bs-9357R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Proteasome 20S beta 3 Antibody, Cy5 Conjugated (bs-9357R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9357R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Proteasome 20S beta 3 Antibody, Cy5 Conjugated (bs-9357R-Cy5)?
KLH conjugated synthetic peptide derived from human Proteasome 20S beta 3
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9357R-Cy5)?
Cy5 on bs-9357R-Cy5: excitation / emission 625,650nm/670nm.