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Proteasome 20S beta 3 Antibody, PE Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-9357R-PE
Product Name Proteasome 20S beta 3 Antibody, PE Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Proteasome 20S beta 3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5684
Synonyms 20S Proteasome _3; C10 II; HC10 II; proteasome prosome, macropain subunit, beta type 3; Proteasome beta 3 subunit; Proteasome chain 13; proteasome component C10 II; Proteasome theta chain; PSMB3.
Background The proteasome is a multicatalytic proteinase complex with a highly ordered ring shaped 20S core structure. The core structure is composed of 4 rings of 28 non identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin dependent process in a non lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.
Application Dilution
WB 1:300-5000
IF

Frequently asked questions

Which applications is Proteasome 20S beta 3 Antibody, PE Conjugated (bs-9357R-PE) validated for, and at what dilution?

bs-9357R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Proteasome 20S beta 3 Antibody, PE Conjugated (bs-9357R-PE) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-9357R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Proteasome 20S beta 3 Antibody, PE Conjugated (bs-9357R-PE)?

KLH conjugated synthetic peptide derived from human Proteasome 20S beta 3

What are the excitation and emission wavelengths of the PE conjugate (bs-9357R-PE)?

PE on bs-9357R-PE: excitation / emission 496,564nm/578nm.