PSMD6 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-9367R-BF488-100uL |
| Product Name | PSMD6 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PSMD6/Proteasome regulatory particle subunit p44S10 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9861 |
| Synonyms | 26S proteasome non-ATPase regulatory subunit 6; 26S proteasome regulatory subunit RPN7; 26S proteasome regulatory subunit S10; Breast cancer-associated protein SGA-113M; KIAA0107; p42A; P44S10; PFAAP4; PSMD6_HUMAN; Phosphonoformate immuno-associated protein 4; Proteasome prosome, macropain 26S subunit, non-ATPase, 6; Proteasome regulatory particle subunit p44S10; Rpn7; S10 antibody SGA-113M. |
| Background | In eukaryotic cells, selective breakdown of cellular proteins is ensured by two distinct pathways, ubiquitination and degradation by the 26S proteasome. At specific stages of development, embryo- and tissue-specific components of the 26S proteasome are formed by developmentally regulated alternative splicing, including Rpn10a through Rpn10e (also designated pUb-R2 through pUb-R5). The pUb-R2 subunit, originally identified as S5a, is ubiquitously expressed and may perform proteolysis constitutively in a wide variety of cells. p44S10 is a highly conserved proteasome regulatory subunit that is expressed in heart, liver, skeletal muscle and pancreas. In addition to normal tissue expression, p44S10 is also expressed in several melanoma cell lines, such as MCF-7, 451Lu and WM164. Since forced expression of p44S10 in radial growth phase melanoma cells results in an increase in cellular proliferation, p44S10 may represent a potential link between regulation of proteasome activity and tumor cell proliferation in vivo. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is PSMD6 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9367R-BF488) validated for, and at what dilution?
bs-9367R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-9367R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PSMD6 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9367R-BF488)?
KLH conjugated synthetic peptide derived from human PSMD6/Proteasome regulatory particle subunit p44S10
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-9367R-BF488)?
AbBy Fluor-488 on bs-9367R-BF488: excitation / emission 499nm/519nm.