MURF3 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9376R-FITC-100uL |
| Product Name | MURF3 Polyclonal Antibody, FITC Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MURF3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 57159 |
| Synonyms | MURF 3; MURF; MURF-3; MuRF3; Muscle specic RING finger protein 3; Muscle specic RING finger protein; Muscle specic RING finger protein homolog; Muscle-specic RING finger protein 3; Muscle-specic RING finger protein; Ring finger protein 30; RNF30; TRI54_HUMAN; TRIM 54; TRIM54; RNF28; Tripartite mot containing 54; tibody Tripartite mot-containing protein 54. |
| Background | MuRF1, is a nuclear protein that interacts with SMT3b and the large myofibrillar protein Titin. In muscle cells, MuRF2 (RFN29) regulates gene expression and protein turnover. It localizes to the cytoplasm, but under atrophic conditions it is detected in the nucleus. MuRF2 can form oligomers with various other proteins, including Titin and Myosin. MuRF3, also designated tripartite motif-containing 54 (TRIM54) or ring finger protein 30 (RNF30), interacts with tubulin and stabilizes microtubules duing myotube formation. It is a cytoplasmic protein the localizes to the Z-lines in skeletal muscles, while MuRF2 localizes to the sarcomeric M-band in cardiomyocytes. MuRF3 shares 77% and 65% sequence identity with MuRF1 and MuRF2, respectively. MuRF1-3 share a conserved N-terminal RING domain and zinc-binding B-box motif, and two coiled-coil dimerization motif boxes, in their central regions. |
| Application Dilution | |
| IF | WB1:300-5000 |
Frequently asked questions
Which applications is MURF3 Polyclonal Antibody, FITC Conjugated (bs-9376R-FITC) validated for, and at what dilution?
bs-9376R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MURF3 Polyclonal Antibody, FITC Conjugated (bs-9376R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9376R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MURF3 Polyclonal Antibody, FITC Conjugated (bs-9376R-FITC)?
KLH conjugated synthetic peptide derived from human MURF3
What are the excitation and emission wavelengths of the FITC conjugate (bs-9376R-FITC)?
FITC on bs-9376R-FITC: excitation / emission 494nm/518nm.