RNF62/MKRN2 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9379R-BF647-100uL |
| Product Name | RNF62/MKRN2 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MKRN2/RNF62 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23609 |
| Synonyms | HSPC070_ Makorin 2; Makorin ring finger protein 2; Makorin2; Makorin-2 MKRN 2; RING finger protein 62; RNF 62; RNF62; MKRN2_HUMAN; Probable E3 ubiquitin-protein ligase makorin-2; RING finger protein 62. |
| Background | The Makorins are a family of putative ribonucleoproteins containing two to four C3H zinc fingers that may confer RNA-binding. In addition, they contain a C3HC4 RING zinc finger that allows them to function as E3 ubiquitin ligases. Makorin-2, also known as RNF62, HSPC070 or MKRN2, is a widely expressed, evolutionarily conserved protein with four C3H-type zinc fingers (three at the N-terminus and one at the C-terminus), one RING-type zinc finger and a cysteine and histidine motif similar to that found in Makorin-1. In Xenopus, Makorin-2 functions, via PI 3-kinase/Akt signaling, as a negative regulator of neurogenesis. In humans, Makorin-2 is overexpressed in various cancer cell lines, suggesting a possible role of Makorin-2 in tumor progression. In addition, Makorin-2 is co-expressed with Raf-1 in the same tissues and cell lines. |
| Application Dilution | |
| IF | WB1:300-5000 |
Frequently asked questions
Which applications is RNF62/MKRN2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9379R-BF647) validated for, and at what dilution?
bs-9379R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF62/MKRN2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9379R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9379R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF62/MKRN2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9379R-BF647)?
KLH conjugated synthetic peptide derived from human MKRN2/RNF62
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-9379R-BF647)?
AbBy Fluor-647 on bs-9379R-BF647: excitation / emission 650nm/665nm.