RNF59/MID1 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9380R-BF647-100uL |
| Product Name | RNF59/MID1 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Midline-1/RNF59 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4281 |
| Swiss Prot | O15344 |
| Synonyms | BBBG1; Finger on X and Y mouse homolog of antibody; FXY; GBBB1; MID-1; Mid1; Midin; Midline 1 Opitz/BBB syndrome; Midline 1; Midline 1 ring finger; Midline 1 RING finger protein; Midline-1; Midline1; OGS1; OSX; Putative transcription factor XPRF; RING finger protein 59; RNF59; TRI18; TRI18_HUMAN; TRIM18; Tripartite mot containing protein 18; Tripartite mot protein TRIM18; Tripartite mot-containing protein 18; XPRF; Zinc finger X and Y antibody; ZNFXY. |
| Background | Midline-1 (Tripartite motif-containing protein 18, Putative transcription factor XPRF, RING finger protein 59) is a 667 amino acid protein encoded by the human gene MID1. Midline-1 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain and one RING-type zinc finger. Midline-1 is believed to have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation. It is a cytoplasmic protein found as a homodimer or heterodimer with Midline-2. It also interacts with IGBP1 (Lymphocyte signaling protein A4). Defects in MID1 are the cause of Opitz syndrome type I (OS-I). OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects. OS-I mutations produce proteins with a decreased affinity for microtubules. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is RNF59/MID1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9380R-BF647) validated for, and at what dilution?
bs-9380R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF59/MID1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9380R-BF647) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Sheep, Pig, Horse, Chicken.
How should bs-9380R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF59/MID1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9380R-BF647)?
KLH conjugated synthetic peptide derived from human Midline-1/RNF59
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-9380R-BF647)?
AbBy Fluor-647 on bs-9380R-BF647: excitation / emission 650nm/665nm.