CNOT8 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9388R-FITC |
| Product Name | CNOT8 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Chicken |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CNOT8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9337 |
| Synonyms | CAF 1; CAF 2; CAF1; CAF1 like protein; CAF1-like protein; CAF2; CAL; CALp; CCR4 associated factor 8; CCR4 NOT transcription complex subunit 8; CCR4-associated factor 8; CCR4-NOT transcription complex subunit 8; CNOT 8; Cnot8; CNOT8_HUMAN; hCAF1; PGK promoter directed over production; POP 2; POP2. |
| Background | The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription as well as mRNA degradation. Various subunits within the complex are involved in influencing nuclear hormone receptor activities. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome. CNOT8 (CCR4-NOT transcription complex subunit 8), also known as CALIF or POP2, is a 292 amino acid protein that localizes to both the nucleus and the cytoplasm and functions as part of the CCR-NOT complex. Expressed ubiquitously, CNOT8 plays a role in transcriptional regulation for a diverse set of processes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is CNOT8 Polyclonal Antibody, FITC Conjugated (bs-9388R-FITC) validated for, and at what dilution?
bs-9388R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CNOT8 Polyclonal Antibody, FITC Conjugated (bs-9388R-FITC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Chicken.
How should bs-9388R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CNOT8 Polyclonal Antibody, FITC Conjugated (bs-9388R-FITC)?
KLH conjugated synthetic peptide derived from human CNOT8
What are the excitation and emission wavelengths of the FITC conjugate (bs-9388R-FITC)?
FITC on bs-9388R-FITC: excitation / emission 494nm/518nm.