SMURF1 Polyclonal Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with SMURF1 polyclonal antibody, unconjugated (bs-9391R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with SMURF1 polyclonal antibody, unconjugated (bs-9391R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Predicted Reactivity

Mouse

Overview
Catalog #	bs-9391r-100l
Product Name	SMURF1 Polyclonal Antibody
Applications	WB
Reactivity	Human
Predicted Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SMURF1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	57154
Synonyms	Smad ubiquitination regulatory factor 1; Smad ubiquitination regulatory factor 1; Smad specic E3 ubiquitin ligase 1; E3 ubiquitin-protein ligase SMURF1; hSMURF1; Smad specic E3 ubiquitin ligase 1; SMAD specic E3 ubiquitin protein ligase 1; Smad ubiquitination regulatory factor 1; SMAD-specic E3 ubiquitin-protein ligase 1; SMUF1_HUMAN; SMURF 1; smurf1.
Background	E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
Application Dilution
WB	1:500-2000