p150 CAF1 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9392R-BF647-100uL |
| Product Name | p150 CAF1 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human p150 CAF1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10036 |
| Synonyms | CAF-1 p150; CAF 1 150 kDa subunit; CAF 1; CAF 1 subunit A; CAF; CAF I 150 kDa subunit; CAF I p150; CAF Ip150; CAF-1 subunit A; CAF-I 150 kDa subunit; CAF-I p150; CAF1 150 kDa subunit; CAF1; CAF1 p150 Subunit; CAF1 subunit A; CAF1A; CAF1A; CAF1A_HUMAN; CAF1B; CAF1P150; CAF1P155; CAF1P155; CHAF1A; Chromatin assembly factor 1 subunit A; Chromatin Assembly Factor 1 Subunit A p150; Chromatin assembly factor I 150 kDa; Chromatin assembly factor I 150 kDa; Chromatin Assembly Factor I; Chromatin assembly factor I p150 subunit; DCAF1; hp150; Nucleosome Remodeling Factor 150kDa Subunit; NURF150; P150. |
| Background | Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is p150 CAF1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9392R-BF647) validated for, and at what dilution?
bs-9392R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does p150 CAF1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9392R-BF647) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse.
How should bs-9392R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise p150 CAF1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9392R-BF647)?
KLH conjugated synthetic peptide derived from human p150 CAF1
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-9392R-BF647)?
AbBy Fluor-647 on bs-9392R-BF647: excitation / emission 650nm/665nm.