CAF1p55/VPRBP Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9393R-Cy5.5 |
| Product Name | CAF1p55/VPRBP Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CAF1p55 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9730 |
| Synonyms | Caf 1; CAF-1; CAF-1 P55 subunit; CAF1 p55 subunit; CAF1A; CAF1P55; chaf1b; Chromatin assembly factor 1 p55 subunit; Chromatin assembly factor 1 p60 subunit; chromatin assembly factor 1 subunit; Chromatin assembly factor I; dCAF-1; dCAF1; Human chromatin assembly factor-I p55 subunit; Nucleosome remodeling factor 55 kDa subunit; NURF 55; NURF-55; p55; Probable histone binding protein Caf1; VPRBP_HUMAN. |
| Background | P55 is an extensively palmitoylated erythrocyte membrane protein, and a member of the MAGUK family. P55 also resists salt extraction, resulting in a high affinity for the plasma membrane. P55 contains a PDZ/DHR domain, a conserved SH-3 domain that appears to suppress tyrosine kinase activity of various oncoproteins, a 39-amino acid motif that binds to cytoskeletal protein 4.1R, and a guanylate kinase-like domain. Interaction with glycophorin C (GPC) and 4.1R suggests that p55 may play a role in the dynamic regulation in the erythrocyte membrane. In addition, p55 gene expression in vivo may be associated with a CpG island. P55 is constitutively expressed in K562 erythroleukemia cells during erythropoiesis and undergoes a 2-fold amplification after induction. |
| Application Dilution | |
| IF | WB1:300-5000 |
Frequently asked questions
Which applications is CAF1p55/VPRBP Polyclonal Antibody, Cy5.5 Conjugated (BS-9393R-CY5-5) validated for, and at what dilution?
BS-9393R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CAF1p55/VPRBP Polyclonal Antibody, Cy5.5 Conjugated (BS-9393R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-9393R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CAF1p55/VPRBP Polyclonal Antibody, Cy5.5 Conjugated (BS-9393R-CY5-5)?
KLH conjugated synthetic peptide derived from human CAF1p55
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-9393R-CY5-5)?
Cy5.5 on BS-9393R-CY5-5: excitation / emission 675nm/694nm.