ANKRD33B Polyclonal Antibody

Lane 1: mouse erebellum lysates; Lane 2: mouse hippocampus lysates probed with ANKRD33B Polyclonal Antibody, Unconjugated (bs-9397R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Rat cerebral cortex lysates probed with ANKRD33B Polyclonal Antibody, Unconjugated (bs-9397R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANKRD33B Polyclonal Antibody, Unconjugated (bs-9397R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANKRD33B Polyclonal Antibody, Unconjugated (bs-9397R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-9397R
Product Name	ANKRD33B Polyclonal Antibody
Applications	WB, FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ANKRD33B
Immunogen Range	401-494/494
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	651746
Swiss Prot	A6NCL7
Synonyms	AN33B_HUMAN; Ankrd33b; Ankyrin repeat domain-containing protein 33B; RGD1564227.
Background	ANKRD33B
Application Dilution
WB	1:300-5000
FCM	1:20-100

Applications

Reactivity

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