OIF Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9407R-BF488-100uL |
| Product Name | OIF Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human OIF |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4969 |
| Synonyms | Corneal keratan sulfate proteoglycan; MIME_HUMAN; mimecan; Mimecan proteoglycan; OG; OGN; O; osteoglycin; osteoinductive factor; SLRR3A. |
| Background | The small leucine-rich proteoglycan (SLRP) family of proteins contains various proteins such as Decorin, Biglycan, Fibromodulin, Keratocan, Lumican, Osteoadherin and Osteoglycin. These proteins all have similar functions as they all mediate extracellular matrix organization and act as binding partners of TGF beta. Osteoglycin, which also may be designated osteoinductive factor (OIF), is a secreted protein detected in bone tissues. Osteoglycin induces the formation of bone in conjunction with either TGF-beta-1 or TGF-beta-2. The precursor form of the OGN gene product, designated Mimecan, is subject to in situ proteolytic cleavage to yield the mature Osteoglycin. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is OIF Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9407R-BF488) validated for, and at what dilution?
bs-9407R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does OIF Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9407R-BF488) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Pig, Horse, Rabbit.
How should bs-9407R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise OIF Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9407R-BF488)?
KLH conjugated synthetic peptide derived from human OIF
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-9407R-BF488)?
AbBy Fluor-488 on bs-9407R-BF488: excitation / emission 499nm/519nm.