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OIF Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • IHC-P

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-9407R-HRP-100uL
Product Name OIF Polyclonal Antibody, HRP Conjugated
Applications
WB IHC-P
Reactivity Rat
Predicted Reactivity Human, Mouse, Cow, Pig, Horse, Rabbit
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human OIF
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4969
Synonyms Corneal keratan sulfate proteoglycan; MIME_HUMAN; mimecan; Mimecan proteoglycan; OG; OGN; O; osteoglycin; osteoinductive factor; SLRR3A.
Background The small leucine-rich proteoglycan (SLRP) family of proteins contains various proteins such as Decorin, Biglycan, Fibromodulin, Keratocan, Lumican, Osteoadherin and Osteoglycin. These proteins all have similar functions as they all mediate extracellular matrix organization and act as binding partners of TGF beta. Osteoglycin, which also may be designated osteoinductive factor (OIF), is a secreted protein detected in bone tissues. Osteoglycin induces the formation of bone in conjunction with either TGF-beta-1 or TGF-beta-2. The precursor form of the OGN gene product, designated Mimecan, is subject to in situ proteolytic cleavage to yield the mature Osteoglycin.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400

Frequently asked questions

Which applications is OIF Polyclonal Antibody, HRP Conjugated (bs-9407R-HRP) validated for, and at what dilution?

bs-9407R-HRP is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does OIF Polyclonal Antibody, HRP Conjugated (bs-9407R-HRP) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Pig, Horse, Rabbit.

How should bs-9407R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise OIF Polyclonal Antibody, HRP Conjugated (bs-9407R-HRP)?

KLH conjugated synthetic peptide derived from human OIF