TSPAN3 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9412R-Cy5 |
| Product Name | TSPAN3 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TSPAN3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10099 |
| Subcellular location | Extracellular |
| Synonyms | tetraspanin 3; tetraspanin TM4 A; TM4 A; TM4A; TM4SF8; transmembrane 4 superfamily member 8; TSPAN 3; TSPAN-3; TSPAN3; TSN3_HUMAN. |
| Background | TSPAN3 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The use of alternate polyadenylation sites has been found for this gene. Two alternative transcripts encoding different isoforms have been described. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is TSPAN3 Polyclonal Antibody, Cy5 Conjugated (bs-9412R-Cy5) validated for, and at what dilution?
bs-9412R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TSPAN3 Polyclonal Antibody, Cy5 Conjugated (bs-9412R-Cy5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, Zebrafish.
How should bs-9412R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TSPAN3 Polyclonal Antibody, Cy5 Conjugated (bs-9412R-Cy5)?
KLH conjugated synthetic peptide derived from human TSPAN3
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9412R-Cy5)?
Cy5 on bs-9412R-Cy5: excitation / emission 625,650nm/670nm.