Help

TSPAN4 Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Sheep
  • Pig
  • Chicken
Overview
Catalog # bs-9413R-Cy5.5
Product Name TSPAN4 Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF(IHC-P)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Sheep, Pig, Chicken
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TSPAN4
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7106
Subcellular location Extracellular
Synonyms AG 2; NAG-2; NAG2; Novel antigen 2; Tetraspan; Tetraspan TM4SF; Tetraspanin 4; Tetraspanin-4; Tetraspanin4; TM4SF7; TM4SF 7; TM4SF-7; Transmembrane 4 superfamily member 7; TSN4_HUMAN; TSPAN 4; Tspan-4; TSPAN4.
Background The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein and is similar in sequence to its family member CD53 antigen. It is known to complex with integrins and other transmembrane 4 superfamily proteins. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is TSPAN4 Polyclonal Antibody, Cy5.5 Conjugated (BS-9413R-CY5-5) validated for, and at what dilution?

BS-9413R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TSPAN4 Polyclonal Antibody, Cy5.5 Conjugated (BS-9413R-CY5-5) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Chicken.

How should BS-9413R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TSPAN4 Polyclonal Antibody, Cy5.5 Conjugated (BS-9413R-CY5-5)?

KLH conjugated synthetic peptide derived from human TSPAN4

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-9413R-CY5-5)?

Cy5.5 on BS-9413R-CY5-5: excitation / emission 675nm/694nm.