Tal1 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9428R-RBITC-100uL |
| Product Name | Tal1 Polyclonal Antibody, RBITC Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Tal1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | bHLHa17; Class A basic helix-loop-helix protein 17; SCL antibody; Stem cell protein; T cell acute lymphocytic leukemia 1; T cell acute lymphocytic leukemia 1 protein; T cell leukemia/lymphoma 5 protein; T-cell acute lymphocytic leukemia protein 1; T-cell leukemia/lymphoma protein 5; Tal 1; Tal 1 product; TAL 1 protein; TAL-1; tal1; TAL1_HUMAN; TCL 5; TCL5. |
| Background | Activation of TAL1 characterizes up to 60% of cases of human T cell acute lymphoblastic leukemia, making it the most frequent gain-of-function mutation observed in this disorder. TAL1 (also designated SCL) is a serine phosphoprotein and basic helix-loop-helix transcription factor known to regulate embryonic hematopoiesis. This transcription factor binds as a heterodimer with E2A and HEB/HTF4 to a nucleotide sequence motif termed the E-box. In addition, leukemogenesis is accelerated dramatically by transgenic co-expression of TAL1 and the catalytic subunit of casein kinase IIalpha, a serine/ threonine protein kinase known to modulate the activity of other bHLH transcription factors. |
| Application Dilution | |
| IF | WB1:300-5000 |
Frequently asked questions
Which applications is Tal1 Polyclonal Antibody, RBITC Conjugated (bs-9428R-RBITC) validated for, and at what dilution?
bs-9428R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Tal1 Polyclonal Antibody, RBITC Conjugated (bs-9428R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9428R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Tal1 Polyclonal Antibody, RBITC Conjugated (bs-9428R-RBITC)?
KLH conjugated synthetic peptide derived from human Tal1
What are the excitation and emission wavelengths of the RBITC conjugate (bs-9428R-RBITC)?
RBITC on bs-9428R-RBITC: excitation / emission 540nm/625nm.