PAPOL A+B+G Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9435R-Cy5.5 |
| Product Name | PAPOL A+B+G Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PAPOLA/PAPOLB/PAPOLG |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10914, 56903, 64895 |
| Swiss Prot | P51003, Q9NRJ5, Q9BWT3 |
| Subcellular location | Cytoplasm, Nucleus, Lumenal |
| Synonyms | PAPOL A/B/G; PAP alpha/beta/gamma; PapIII; MGC5378; PAP alpha; PAP beta; PAP gamma; PAP; PAP-alpha; PAP-beta; PAP-gamma; PAPOLA; PAPOLB; PAPOLG; PAPOA_HUMAN; PAPOB_HUMAN; PAPOG_HUMAN; Plap; polyA polymerase alpha; Polynucleotide adenylyltransferase alpha; polyA polymerase beta testis specic; polyA polymerase beta; polynucleotide adenylyltransferase beta; TPAP; Testis-specic polyA polymerase. |
| Background | Polyadenylation of the 3-prime ends of eukaryotic mRNAs is a key event that takes place in the nucleus during maturation of mRNA. The reaction includes endoribonucleolytic cleavage of the pre-RNA at the poly(A) site that leads to synthesis of the poly(A) tail at the 3-prime end of the upstream cleavage product. The poly(A) polymerase (PAP) is required The adenosine addition reaction depends on poly(A) polymerase (PAP) activity. The testis express PAP-beta (TPAP) in the cytoplasm of spermatogenic cells. The adenosine addition function of PAP-beta plays a critical role in male germ cell production. PAP-beta-deficient transgenic mice display impaired expression of haploid-specific genes that are necessary for spermatogenesis. The intronless gene encoding human PAP-beta maps to chromosome 7p22.3. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is PAPOL A+B+G Polyclonal Antibody, Cy5.5 Conjugated (BS-9435R-CY5-5) validated for, and at what dilution?
BS-9435R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PAPOL A+B+G Polyclonal Antibody, Cy5.5 Conjugated (BS-9435R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-9435R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PAPOL A+B+G Polyclonal Antibody, Cy5.5 Conjugated (BS-9435R-CY5-5)?
KLH conjugated synthetic peptide derived from human PAPOLA/PAPOLB/PAPOLG
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-9435R-CY5-5)?
Cy5.5 on BS-9435R-CY5-5: excitation / emission 675nm/694nm.