PRKAG2 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9446R-BF680-100uL |
| Product Name | PRKAG2 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PRKAG2/AMPK_2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5571, 51422, 53632, |
| Subcellular location | Extracellular, Cytoplasm, Nucleus |
| Synonyms | 5'-AMP-activated protein kinase subunit gamma-2; AAKG; AMPK gamma2; AMPK gamma 2; AMPK subunit gamma 2; CMH6; H91620p; AMPKg; AMPK subunit gamma-1; AMPK gamma1; AMPKg3; AMPK subunit gamma-3; AMPK gamma3. |
| Background | AMPK is a heterotrimeric complex comprising a catalytic a subunit and regulatory b and g subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKa1 and AMPKa2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKb1 encodes a 271 amino acid protein and human AMPKb2 encodes a 272 amino acid protein. The human AMPKg1 gene encodes a 331 amino acid protein. Human AMPKg2 and AMPKg3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is PRKAG2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-9446R-BF680) validated for, and at what dilution?
bs-9446R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PRKAG2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-9446R-BF680) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse, Chicken.
How should bs-9446R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PRKAG2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-9446R-BF680)?
KLH conjugated synthetic peptide derived from human PRKAG2/AMPK_2
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-9446R-BF680)?
AbBy Fluor-680 on bs-9446R-BF680: excitation / emission 679nm/702nm.