BMP10 Polyclonal Antibody, RBITC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-9447R-RBITC-100uL |
| Product Name | BMP10 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BMP10 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27302 |
| Swiss Prot | O95393 |
| Subcellular location | Secreted |
| Synonyms | Bone morphogenetic protein 1; BMP-1; BMP1 |
| Background | Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. May reduce cell migration and cell matrix adhesion in breast cancer cell lines. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is BMP10 Polyclonal Antibody, RBITC Conjugated (bs-9447R-RBITC) validated for, and at what dilution?
bs-9447R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-9447R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BMP10 Polyclonal Antibody, RBITC Conjugated (bs-9447R-RBITC)?
KLH conjugated synthetic peptide derived from human BMP10
What are the excitation and emission wavelengths of the RBITC conjugate (bs-9447R-RBITC)?
RBITC on bs-9447R-RBITC: excitation / emission 540nm/625nm.