5 MethylCytosine Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9450R-BF680 |
| Product Name | 5 MethylCytosine Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
|
| Reactivity | Human, Others |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated 5 MethylCytosine |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | 5-Methyl Cytidine; 5 m C; 5 mC; 5 me C; 5 Me Cytidine; 5 MeCyd; 5 Methyl Cytidine; 5 MethylCytosine; 5-mC; 5-Me Cytidine; 5-Methyl-Cytosine; 5-MethylCytidine. |
| Background | Cytidine is a nucleoside formed by a cytosine attached to a ribose ring via a beta-N1-glycosidic bond. DNA is methylated on cytidines by DNA methylases (DNMTs)to generate 5-methylcytidine (5-mC), a potent epigenetics marker and regulator of gene expression. The reverse reaction (cytidine demethylation) starts with its oxidation to hydroxymethyl- (5-hmC), formyl- (5-fC), and carboxy- (5-caC) cytidine. Several enzymes, including the Tet family of proteins have been implicated in cytidine demethylation. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is 5 MethylCytosine Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-9450R-BF680) validated for, and at what dilution?
bs-9450R-BF680 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does 5 MethylCytosine Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-9450R-BF680) react with?
Tested reactivity: Human, Others.
How should bs-9450R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise 5 MethylCytosine Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-9450R-BF680)?
KLH conjugated 5 MethylCytosine
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-9450R-BF680)?
AbBy Fluor-680 on bs-9450R-BF680: excitation / emission 679nm/702nm.