FOG1 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9451R-BF405 |
| Product Name | FOG1 Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FOG1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 161882 |
| Synonyms | FOG-1; FOG1_HUMAN; Friend of GATA 1; Friend of GATA protein 1; Friend of GATA1; ZFPM1; Zinc finger protein; Zinc finger protein 89A; Zinc finger protein multitype 1; Zinc finger protein ZFPM1. |
| Background | Transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. May be involved in regulation of some genes in gonads. May also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is FOG1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-9451R-BF405) validated for, and at what dilution?
bs-9451R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FOG1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-9451R-BF405) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9451R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FOG1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-9451R-BF405)?
KLH conjugated synthetic peptide derived from human FOG1
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-9451R-BF405)?
AbBy Fluor-405 on bs-9451R-BF405: excitation / emission 401nm/421nm.