GPBB Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9453R-APC-Cy7 |
| Product Name | GPBB Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GPBB/PYGB |
| Immunogen Range | 301-400/843 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5834 |
| Synonyms | Brain glycogen phosphorylase; Glycogen phosphorylase B; Glycogen phosphorylase brain form; Glycogen Phosphorylase Isoenzyme BB; Glycogen phosphorylase, brain form; MGC9213; Phosphorylase glycogen brain; PYGB; PYGB_HUMAN. |
| Background | Glycolysis is an evolutionarily conserved series of ten chemical reactions that utilizes eleven enzymes to concomitantly generate pyruvate and ATP from glucose. fructose kinase-2/fructose 2,6-bisphosphatase (PFK-2) stimulates the synthesis and degradation of fructose 2,6-bisphosphate. Glycogen phosphorylase (also known as GP) is an allosteric enzyme important in carbohydrate metabolism. Its activity is regulated through either noncovalent binding of metabolites or by covalent modification. Glycogen phosphorylase catalyzes the phosphorylation of glycogen to Glc-1-P. There are three genes which encode the brain, liver and muscle forms of glycogen phosphorylase, PYGB, PYGL and PYGM. Because of its fundamental role in the metabolism of glycogen, glycogen phosphorylase has been a target for the design of inhibitory compounds, which could be valuable in the therapeutic treatment of type 2 diabetes mellitus. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is GPBB Polyclonal Antibody, APC-Cy7 Conjugated (bs-9453R-APC-Cy7) validated for, and at what dilution?
bs-9453R-APC-Cy7 is validated for IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GPBB Polyclonal Antibody, APC-Cy7 Conjugated (bs-9453R-APC-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9453R-APC-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GPBB Polyclonal Antibody, APC-Cy7 Conjugated (bs-9453R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human GPBB/PYGB Immunogen range: 301-400/843.
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-9453R-APC-Cy7)?
APC-Cy7 on bs-9453R-APC-Cy7: excitation / emission 650nm/780nm.