GPBB Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9453R-Biotin-100uL |
| Product Name | GPBB Polyclonal Antibody, Biotin Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GPBB/PYGB |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 5834 |
| Synonyms | Brain glycogen phosphorylase; Glycogen phosphorylase B; Glycogen phosphorylase brain form; Glycogen Phosphorylase Isoenzyme BB; Glycogen phosphorylase, brain form; MGC9213; Phosphorylase glycogen brain; PYGB; PYGB_HUMAN. |
| Background | Glycolysis is an evolutionarily conserved series of ten chemical reactions that utilizes eleven enzymes to concomitantly generate pyruvate and ATP from glucose. fructose kinase-2/fructose 2,6-bisphosphatase (PFK-2) stimulates the synthesis and degradation of fructose 2,6-bisphosphate. Glycogen phosphorylase (also known as GP) is an allosteric enzyme important in carbohydrate metabolism. Its activity is regulated through either noncovalent binding of metabolites or by covalent modification. Glycogen phosphorylase catalyzes the phosphorylation of glycogen to Glc-1-P. There are three genes which encode the brain, liver and muscle forms of glycogen phosphorylase, PYGB, PYGL and PYGM. Because of its fundamental role in the metabolism of glycogen, glycogen phosphorylase has been a target for the design of inhibitory compounds, which could be valuable in the therapeutic treatment of type 2 diabetes mellitus. |
| Application Dilution | |
| ELISA | IHC-P1:200-400 |
| IHC-P | |
| IHC-F | |
Frequently asked questions
Which applications is GPBB Polyclonal Antibody, Biotin Conjugated (bs-9453R-Biotin) validated for, and at what dilution?
bs-9453R-Biotin is validated for IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GPBB Polyclonal Antibody, Biotin Conjugated (bs-9453R-Biotin) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9453R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GPBB Polyclonal Antibody, Biotin Conjugated (bs-9453R-Biotin)?
KLH conjugated synthetic peptide derived from human GPBB/PYGB