HEXIM1 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9460R-BF350 |
| Product Name | HEXIM1 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HEXIM1/EDG1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10614 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Cardiac lineage protein 1; CLP 1; CLP1; EDG 1; EDG1; Estrogen down-regulated gene 1 protein; Hexamethylene bis acetamide inducible 1; Hexamethylene bis acetamide inducible protein; Hexamethylene bis acetamide inducible transcript 1; Hexamethylene bis-acetamide-inducible protein 1; HEXI1_HUMAN; HEXIM 1; Hexim1; HEXIM1 protein; HIS 1; HIS1; HMBA inducible; MAQ 1; MAQ1; Menage a quatre 1; Menage a quatre protein 1; Protein HEXIM1. |
| Background | Hexamethylene bis-acetamide inducible 1 (HEXIM1) and Hexamethylene bis-acetamide inducible 2 (HEXIM2) comprise a family of proteins which inhibit positive transcription elongation factor b (P-TEFb) through association with 7SK. P-TEFb is composed of a catalytic subunit, Cdk9, and either Cyclin T1 or T2 as a regulatory subunit. This complex regulates eukaryotic gene expression at the level of elongation. The C-terminal domains of HEXIM proteins interact directly with each other. Via these domains, HEXIM1 and HEXIM2 form stable homo- and hetero-oligomers, which may aid in the formation of the 7SK small nuclear ribonucleic acid particle. Despite their similar functions, HEXIM1 and HEXIM2 exhibit distinct expression patterns in various established cell lines and human tissues. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is HEXIM1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-9460R-BF350) validated for, and at what dilution?
bs-9460R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HEXIM1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-9460R-BF350) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Horse.
How should bs-9460R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HEXIM1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-9460R-BF350)?
KLH conjugated synthetic peptide derived from human HEXIM1/EDG1
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-9460R-BF350)?
AbBy Fluor-350 on bs-9460R-BF350: excitation / emission 346nm/442nm.