HEY2 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9461R-BF350 |
| Product Name | HEY2 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HEY2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23493 |
| Synonyms | bHLHb32; Cardiovascular helix loop helix factor 1; Cardiovascular helix-loop-helix factor 1; CHF1; Class B basic helix-loop-helix protein 32; GRIDLOCK; GRL; Hairy and enhancer of split related 2; Hairy and enhancer of split-related protein 2; Hairy related transcription factor 2; Hairy-related transcription factor 2; Hairy/enhancer-of-split related with YRPW mot protein 2; hCHF1; HERP; HERP1; HES related repressor protein 1; HES-related repressor protein 2; HESR-2; HESR2; HEY2; HEY2_HUMAN; hHRT2; HRT-2; HRT2; Protein gridlock homolog. |
| Background | The LIN-12/Notch family of transmembrane receptors plays a central role in development by regulating cell fate and establishing boundaries of gene expression. Notch signaling activates the Hairy/Enhancer of split (HES) genes, which encode basic helix-loop-helix (bHLH) transcriptional repressors that are critical for directing embryonic patterning and development. The Hairy-related transcription factors (HRTs) comprise a subclass of bHLH proteins that exhibit structural similarity with the HES proteins and include HRT1, HRT2 and HRT3. The HRT family (also designated Hesr, Hey, CHF and Gridlock) contain a bHLH domain, an Orange domain and a novel YRPW domain, which is absent in HRT3. The Hairy-related genes map to human chromosomes 8q21, 6q21 and 1p34.3 for HRT1, HRT2 and HRT3, respectively, and are downstream targets for Notch signaling. HRT1 is expressed in the somitic mesoderm, central nervous system, kidney, heart, nasal epithelium and limb buds in murine embryos as well as in adult tissues. It has altered expression in many breast, lung and kidney tumors. Like HRT1, HRT2 and HRT3 are also expressed in developing somites, heart and nervous system. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is HEY2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-9461R-BF350) validated for, and at what dilution?
bs-9461R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HEY2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-9461R-BF350) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9461R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HEY2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-9461R-BF350)?
KLH conjugated synthetic peptide derived from human HEY2
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-9461R-BF350)?
AbBy Fluor-350 on bs-9461R-BF350: excitation / emission 346nm/442nm.