Nebulette Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9468R-Cy5 |
| Product Name | Nebulette Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Nebulette |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10529 |
| Swiss Prot | O76041 |
| Subcellular location | Cytoplasm |
| Synonyms | LASP2; LNEBL; Nebulette; Actin-binding Z-disk protein; NEBL |
| Background | Binds to actin and plays an important role in the assembly of the Z-disk. Isoform 2 might play a role in the assembly of focal adhesion. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is Nebulette Polyclonal Antibody, Cy5 Conjugated (bs-9468R-Cy5) validated for, and at what dilution?
bs-9468R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Nebulette Polyclonal Antibody, Cy5 Conjugated (bs-9468R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9468R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Nebulette Polyclonal Antibody, Cy5 Conjugated (bs-9468R-Cy5)?
KLH conjugated synthetic peptide derived from human Nebulette
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9468R-Cy5)?
Cy5 on bs-9468R-Cy5: excitation / emission 625,650nm/670nm.