MEF2B Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9470R-BF647 |
| Product Name | MEF2B Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MEF2B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | PRDM3; MADS box transcription enhancer factor 2; MDS1 EVI1; Mef2b; MEF2B_HUMAN; MEF-2B; myocyte enhancer factor 2B; myocyte specic enhancer factor 2B; Myocyte-specic enhancer factor 2B; RSRFR2; serum response factor like protein 2; Serum response factor-like protein 2; XMEF2. |
| Background | The myocyte enhancer factor-2 (MEF-2) family of transcription factors associate with co-repessors or co-activators to regulate development and function of T cells, neuronal cells, and muscle cells. Four family members, termed MEF-2A, -2B, -2C, and -2D, arise from alternatively spliced transcripts. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each during embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5, and MRF4, are one class of identified factors. The MEF-2 family represents a second class of DNA binding regulatory proteins. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is MEF2B Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9470R-BF647) validated for, and at what dilution?
bs-9470R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MEF2B Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9470R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9470R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MEF2B Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9470R-BF647)?
KLH conjugated synthetic peptide derived from human MEF2B
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-9470R-BF647)?
AbBy Fluor-647 on bs-9470R-BF647: excitation / emission 650nm/665nm.