phospho-NFAT5 (Ser1197) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9474R-Cy5-100uL |
| Product Name | phospho-NFAT5 (Ser1197) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human NFAT5 around the phosphorylation site of Ser1197 |
| Modification Site | Ser1197 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10725 |
| Synonyms | NFAT5 phospho S1197; p-NFAT5phospho S1197; Glutamine rich protein H65; KIAA0827; NF AT5; NF-AT5; NFAT 5; NFAT L1; NFAT like protein 1; NFAT5; NFAT5_HUMAN; NFATL 1; NFATL1; NFATZ; Nuclear factor of activated T cells 5; Nuclear factor of activated T cells 5 tonicity responsive; Nuclear factor of activated T cells; Nuclear factor of activated T-cells 5; OREBP; Osmotic response element binding protein; T cell transcription factor NFAT 5; T cell transcription factor NFAT5; T-cell transcription factor NFAT5; TonE binding protein; TonE-binding protein ; TonEBP; Tonicity responsive enhancer binding protein; Tonicity-responsive enhancer-binding protein. |
| Background | Members of the NFAT (nuclear factor of activated T cells) family of transcription factors are related to NFkB/Rel proteins and form cooperative complexes with the AP-1 proteins, Fos and Jun, on DNA to regulate cytokine expression in T cells. NFAT proteins are widely expressed and alternatively modified to generate splice variants, and they are localized to both the cytosol (NFATc) and to the nucleus (NFATn). NFAT1, NFAT2, and NFAT4 are predominantly expressed in immune cells, and NFAT2 and NFAT3 are expressed at high levels in cardiac tissues. In addition to activating cytokine gene transcription, NFAT2 is also implicated in cardiac valve development, and NFAT3 is involved in cardiac hypertrophy. NFAT5 is detected in both immune and nonimmune cells and, like other NFAT proteins, contains a highly conserved Rel-like binding domain that mediates NFAT proteins associating with specific consensus sequences on DNA. NFAT proteins are activated by increases in intracellular calcium, which leads to the calmodulin-dependent phosphatase, calcineurin, dephosphorylating NFAT proteins. This activating event induces a conformational change in the protein structure that exposes the nuclear localization signal and facilitates the translocation of NFAT proteins from the cytosol into the nucleus. |
| Application Dilution | |
| IF | WB1:300-5000 |
Frequently asked questions
Which applications is phospho-NFAT5 (Ser1197) Polyclonal Antibody, Cy5 Conjugated (bs-9474R-Cy5) validated for, and at what dilution?
bs-9474R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-NFAT5 (Ser1197) Polyclonal Antibody, Cy5 Conjugated (bs-9474R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9474R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-NFAT5 (Ser1197) Polyclonal Antibody, Cy5 Conjugated (bs-9474R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human NFAT5 around the phosphorylation site of Ser1197
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9474R-Cy5)?
Cy5 on bs-9474R-Cy5: excitation / emission 625,650nm/670nm.