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C5orf51 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded rat liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C5orf51 Polyclonal Antibody, Unconjugated (bs-9484R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C5orf51 Polyclonal Antibody, Unconjugated (bs-9484R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
LoVo cell lysates probed with C5orf51 Polyclonal Antibody, Unconjugated (bs-9484R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
LoVo cell lysates probed with C5orf51 Polyclonal Antibody, Unconjugated (bs-9484R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-9484R
Product Name C5orf51 Polyclonal Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human C5orf51
Immunogen Range 1-100/294
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms Chromosome 5 open reading frame 51; Hypothetical protein LOC285636; UPF0600 protein C5orf51; CE051_HUMAN.
Background With 181 million base pairs encoding around 1,000 genes, chromosome 5 is about 6% of human genomic DNA. It is associated with Cockayne syndrome through the ERCC8 gene and familial adenomatous polyposis through the adenomatous polyposis coli (APC) tumor suppressor gene. Treacher Collins syndrome is also chromosome 5 associated and is caused by insertions or deletions within the TCOF1 gene. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome. Deletion of 5q or chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. The LOC285636 gene product has been provisionally designated LOC285636 pending further characterization.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F IFIHC-P1:50-200
IF

Frequently asked questions

Which applications is C5orf51 Polyclonal Antibody (bs-9484R) validated for, and at what dilution?

bs-9484R is validated for WB (1:300-5000); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does C5orf51 Polyclonal Antibody (bs-9484R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-9484R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise C5orf51 Polyclonal Antibody (bs-9484R)?

KLH conjugated synthetic peptide derived from human C5orf51 Immunogen range: 1-100/294.

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