Factor XIIa heavy chain Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9503R-BF594 |
| Product Name | Factor XIIa heavy chain Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Coagulation factor XIIa heavy chain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2161 |
| Synonyms | Factor XII; Coagulation factor XII; Factor XII heavy chain; Coagulation factor XIIa heavy chain; F12; F12 deficiency; FA12_HUMAN; Factor XII deficiency; HAE3; HAEX; HAF; HAF deficiency; Hageman factor; Coagulation factor XIIa heavy chain. |
| Background | This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is Factor XIIa heavy chain Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-9503R-BF594) validated for, and at what dilution?
bs-9503R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Factor XIIa heavy chain Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-9503R-BF594) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9503R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Factor XIIa heavy chain Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-9503R-BF594)?
KLH conjugated synthetic peptide derived from human Coagulation factor XIIa heavy chain
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-9503R-BF594)?
AbBy Fluor-594 on bs-9503R-BF594: excitation / emission 590nm/617nm.