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AHSP Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
Overview
Catalog # bs-9518R-Cy3
Product Name AHSP Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF
Reactivity Human
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human AHSP/ERAF
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Synonyms AHSP; AHSP_HUMAN; Alpha hemoglobin stabilizing protein; Alpha-hemoglobin-stabilizing protein; EDRF; ERAF; Erythroid associated factor; Erythroid dferentiation associated factor; Erythroid dferentiation related factor; Erythroid dferentiation-related factor; Erythroid-associated factor.
Background Act as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia.
Application Dilution
WB 1:300-5000
IF IHC-P1:50-200

Frequently asked questions

Which applications is AHSP Polyclonal Antibody, Cy3 Conjugated (bs-9518R-Cy3) validated for, and at what dilution?

bs-9518R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does AHSP Polyclonal Antibody, Cy3 Conjugated (bs-9518R-Cy3) react with?

Tested reactivity: Human.

How should bs-9518R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise AHSP Polyclonal Antibody, Cy3 Conjugated (bs-9518R-Cy3)?

KLH conjugated synthetic peptide derived from human AHSP/ERAF

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9518R-Cy3)?

Cy3 on bs-9518R-Cy3: excitation / emission 512,550nm/570,615nm.