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FECH Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-9521R-Biotin
Product Name FECH Polyclonal Antibody, Biotin Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Human, Mouse, Rat
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human EPB41
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 2235
Synonyms EPP; FCE; Ferrochelatase protoporphyria; Ferrochelatase; Ferrochelatase mitochondrial; Heme synthetase; Protoheme ferro lyase; HEMH_HUMAN.
Background Ferrochelatase catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway, and is localised in the mitochondrion. Defects in ferrochelatase are associated with protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. Porphyrias are a group of inherited or acquired disorders of certain enzymes in the heme biosynthetic pathway (also called porphyrin pathway). They are broadly classified as hepatic porphyrias or erythropoietic porphyrias, based on the site of the overproduction and mainly accumulation of the porphyrins (or their chemical precursors). They manifest with either skin problems, or neurological complications, or occasionally both.
Application Dilution
WB 1:300-5000
ELISA IHC-P1:200-400
IHC-P
IHC-F

Frequently asked questions

Which applications is FECH Polyclonal Antibody, Biotin Conjugated (bs-9521R-Biotin) validated for, and at what dilution?

bs-9521R-Biotin is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FECH Polyclonal Antibody, Biotin Conjugated (bs-9521R-Biotin) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-9521R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FECH Polyclonal Antibody, Biotin Conjugated (bs-9521R-Biotin)?

KLH conjugated synthetic peptide derived from human EPB41