PIGA Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9524R-FITC |
| Product Name | PIGA Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PIGA |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5277 |
| Synonyms | GlcNAc PI synthesis protein; GlcNAc-PI synthesis protein; GPI3; Phosphatidylinositol glycan anchor biosynthesis class A; phosphatidylinositol glycan anchor biosynthesis, class A paroxysmal nocturnal hemoglobinuria; Phosphatidylinositol N-acetylglucosaminyltransferase subunit A; PIG A; PIG-A; PIGA_HUMAN; Phosphatidylinositol-glycan biosynthesis class A protein; PIG-A. |
| Background | PIGA belongs to the glycosyltransferase 1 family and is necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis. Defects in PIGA are the cause of paroxysmal nocturnal hemoglobinuria (PNH) which is an acquired hemolytic blood disorder characterized by chronic hemolysis with hemoglobinuria, increased tendency to venous thrombosis, and variable degrees of bone marrow failure. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is PIGA Polyclonal Antibody, FITC Conjugated (bs-9524R-FITC) validated for, and at what dilution?
bs-9524R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PIGA Polyclonal Antibody, FITC Conjugated (bs-9524R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9524R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PIGA Polyclonal Antibody, FITC Conjugated (bs-9524R-FITC)?
KLH conjugated synthetic peptide derived from human PIGA
What are the excitation and emission wavelengths of the FITC conjugate (bs-9524R-FITC)?
FITC on bs-9524R-FITC: excitation / emission 494nm/518nm.