KLF1, 2, 4 Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9526R-HRP |
| Product Name | KLF1, 2, 4 Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KLF1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10661 |
| Swiss Prot | Q13351 |
| Subcellular location | Nucleus |
| Synonyms | EKLF; INLU; CDAN4; HBFQTL6; Krueppel-like factor 1; Erythroid krueppel-like transcription factor; KLF1 |
| Background | Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is KLF1, 2, 4 Polyclonal Antibody, HRP Conjugated (bs-9526R-HRP) validated for, and at what dilution?
bs-9526R-HRP is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KLF1, 2, 4 Polyclonal Antibody, HRP Conjugated (bs-9526R-HRP) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9526R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KLF1, 2, 4 Polyclonal Antibody, HRP Conjugated (bs-9526R-HRP)?
KLH conjugated synthetic peptide derived from human KLF1