Orai2 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9541R-Cy3 |
| Product Name | Orai2 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Orai2 |
| Immunogen Range | 1-100/254 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 80228 |
| Synonyms | C7orf19; CAP binding protein complex interacting protein 2; CBCIP 2; CBCIP2; Chromosome 7 open reading frame 19; FLJ12474; FLJ14733; FLJ44818; H_NH0514P08.8; Orai 2; ORAI calcium release activated calcium modulator 2; Protein orai 2; TMEM 142B; TMEM142B; Transmembrane protein 142B. |
| Background | Orai2 is a 254 amino acid multi-pass membrane protein that belongs to the orai family of proteins. Localizing to the plasma membrane, Orai2 plays an important role in store-operated calcium (SOC) entry, a process involving Ca2+ influx and replenishment of Ca2+ stores formerly emptied through the action of inositol 1,4,5-trisphosphate production and other Ca2+ mobilizing agents. CRAC channels are responsible for medi-ating calcium influx in T-cells and play an important role in the immune response. Orai2 specifically increases the Ca2+-selective current through coaction with the Ca2+ sensor Stim1. |
| Application Dilution | |
| WB | 1:50-200 |
| IF(IHC-P) | 1:300-5000 |
Frequently asked questions
Which applications is Orai2 Polyclonal Antibody, Cy3 Conjugated (bs-9541R-Cy3) validated for, and at what dilution?
bs-9541R-Cy3 is validated for WB (1:50-200); IF(IHC-P) (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Orai2 Polyclonal Antibody, Cy3 Conjugated (bs-9541R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9541R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Orai2 Polyclonal Antibody, Cy3 Conjugated (bs-9541R-Cy3)?
KLH conjugated synthetic peptide derived from human Orai2 Immunogen range: 1-100/254.
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9541R-Cy3)?
Cy3 on bs-9541R-Cy3: excitation / emission 512,550nm/570,615nm.