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TSPEAR Polyclonal Antibody

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-9577R-100uL
Product Name TSPEAR Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TSPEAR/C21orf9
Immunogen Range 581-669/669
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms C21orf14; C21orf38; C21orf7; Chromosome 21 open reading frame 38; Chromosome 21 open reading frame 91; Early undferentiated retina and lens; EURL; Protein EURL homolog; YG81; TSEAR_HUMAN.
Background TSPEAR, also known as C21orf9, is a 669 amino acid secreted protein. Expressed as two isoforms produced by alternative splicing, TSPEAR contains one Thrombospondin N-terminal domain and seven EAR (epilepsy-associated repeat) domains. EAR domains are found in several proteins, including TSPEAR, encoded by genes that map within chromosome regions associated with seizure disorders. It is thought that the EAR domain plays a role in the pathogenesis of epilepsy by either binding to an unknown epileptic ligand or interfering with axon synaptogenesis.
Application Dilution
WB 1:300-5000
ELISA IHC-P1:200-400
IHC-P IFIHC-P1:50-200
IHC-F
IF
ICC/IF

Frequently asked questions

Which applications is TSPEAR Polyclonal Antibody (bs-9577R) validated for, and at what dilution?

bs-9577R is validated for WB (1:300-5000); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TSPEAR Polyclonal Antibody (bs-9577R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-9577R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TSPEAR Polyclonal Antibody (bs-9577R)?

KLH conjugated synthetic peptide derived from human TSPEAR/C21orf9 Immunogen range: 581-669/669.

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