TSPEAR Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9577R-Cy5 |
| Product Name | TSPEAR Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TSPEAR/C21orf9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | C21orf14; C21orf38; C21orf7; Chromosome 21 open reading frame 38; Chromosome 21 open reading frame 91; Early undferentiated retina and lens; EURL; Protein EURL homolog; YG81; TSEAR_HUMAN. |
| Background | TSPEAR, also known as C21orf9, is a 669 amino acid secreted protein. Expressed as two isoforms produced by alternative splicing, TSPEAR contains one Thrombospondin N-terminal domain and seven EAR (epilepsy-associated repeat) domains. EAR domains are found in several proteins, including TSPEAR, encoded by genes that map within chromosome regions associated with seizure disorders. It is thought that the EAR domain plays a role in the pathogenesis of epilepsy by either binding to an unknown epileptic ligand or interfering with axon synaptogenesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is TSPEAR Polyclonal Antibody, Cy5 Conjugated (bs-9577R-Cy5) validated for, and at what dilution?
bs-9577R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TSPEAR Polyclonal Antibody, Cy5 Conjugated (bs-9577R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9577R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TSPEAR Polyclonal Antibody, Cy5 Conjugated (bs-9577R-Cy5)?
KLH conjugated synthetic peptide derived from human TSPEAR/C21orf9
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9577R-Cy5)?
Cy5 on bs-9577R-Cy5: excitation / emission 625,650nm/670nm.