phospho-NHE3 (Ser552) Polyclonal Antibody

Applications

ELISA
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-9582R
Product Name	phospho-NHE3 (Ser552) Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human NHE-3 around the phosphorylation site of Ser552 [RG(p-S)LA](human Ser555)
Modification Site	Ser552
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms	p-NHE-3; Sodium / Hydrogen Exchanger 3 phospho S552; Na+/H+ exchanger 3; NHE 3; NHE-3; NHE3; SL9A3_HUMAN; SLC9A 3; Slc9a3; Sodium/hydrogen exchanger 3; Sodium/hydrogen exchanger, apical epithelial; Solute carrier family 9 sodium/hydrogen exchanger, isoform 3; Solute carrier family 9 sodium/hydrogen exchanger, member 3; Solute carrier family 9 member 3; MGC126718; MGC126720.
Background	NHE-3 are integral membrane proteins that are expressed in most mammalian tissues, where they regulate intracellular pH and cell volume. NHEs mediate the transport of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+) ions. While NHE-1 is ubiquitously expressed, the NHE isoforms 2-8 have distinct tissue- and cell type-dependent expression and inhibitory characteristics. NHE-3 localizes to the apical membrane of renal proximal tubules where it is responsible for most of the sodium transport and fluid reabsorption. NHE-3 translocates to internal pools where it mediates natriuresis when blood pressure increases abruptly. NHE-3 is also expressed in the stomach and functions to protect the mucosa by secreting protons that diffuse into the mucous cells.
Application Dilution
ELISA	WB1:300-5000
IHC-P	1:200-400
IHC-F	IFIHC-P1:50-200
IF
ICC/IF