gamma crystallin S Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with gamma crystallin S Polyclonal Antibody, Unconjugated (bs-9585R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with gamma crystallin S Polyclonal Antibody, Unconjugated (bs-9585R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Pig
Rabbit

Overview
Catalog #	bs-9585R
Product Name	gamma crystallin S Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Pig, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human gamma crystallin S
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms	rncat; AI327013; Beta-crystallin S; CRBS_HUMAN; CRYG8; crygs; Crystallin, gamma 8; Crystallin, gamma polypeptide 8; Crystallin, gamma S; Gamma crystallin S; Gamma S crystallin; Gamma-crystallin S; Gamma-S-crystallin; recessive nuclear cataract; Opj.
Background	Crystallins are water soluble structural proteins found in the vertebrate eye. Mammalian crystallins are classified in three forms, designated , _ and _. Crystallins, as the principal components of the lens, function to increase the refractive index of the eye during accommodation by forming high-molecular weight aggregates which maintain transparency. _S-crystallin (Gamma-crystallin S), also known as Beta-crystallin S, is a 178 amino acid protein that exists as a monomer which does not aggregate. _S-crystallin contains a two-domain beta structure and belongs to the beta/gamma-crystallin gene family mapping to human chromosome 3. _S-crystallin has been linked to congenital cataract development, a disorder signified by increasing levels of lens opacity.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200