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gamma crystallin S Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Pig
  • Rabbit
Overview
Catalog # bs-9585R-FITC
Product Name gamma crystallin S Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Pig, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human gamma crystallin S
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Synonyms rncat; AI327013; Beta-crystallin S; CRBS_HUMAN; CRYG8; crygs; Crystallin, gamma 8; Crystallin, gamma polypeptide 8; Crystallin, gamma S; Gamma crystallin S; Gamma S crystallin; Gamma-crystallin S; Gamma-S-crystallin; recessive nuclear cataract; Opj.
Background Crystallins are water soluble structural proteins found in the vertebrate eye. Mammalian crystallins are classified in three forms, designated , _ and _. Crystallins, as the principal components of the lens, function to increase the refractive index of the eye during accommodation by forming high-molecular weight aggregates which maintain transparency. _S-crystallin (Gamma-crystallin S), also known as Beta-crystallin S, is a 178 amino acid protein that exists as a monomer which does not aggregate. _S-crystallin contains a two-domain beta structure and belongs to the beta/gamma-crystallin gene family mapping to human chromosome 3. _S-crystallin has been linked to congenital cataract development, a disorder signified by increasing levels of lens opacity.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is gamma crystallin S Polyclonal Antibody, FITC Conjugated (bs-9585R-FITC) validated for, and at what dilution?

bs-9585R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does gamma crystallin S Polyclonal Antibody, FITC Conjugated (bs-9585R-FITC) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Rabbit.

How should bs-9585R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise gamma crystallin S Polyclonal Antibody, FITC Conjugated (bs-9585R-FITC)?

KLH conjugated synthetic peptide derived from human gamma crystallin S

What are the excitation and emission wavelengths of the FITC conjugate (bs-9585R-FITC)?

FITC on bs-9585R-FITC: excitation / emission 494nm/518nm.