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SPAG17 Polyclonal Antibody, PE-Cy5 Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-9596R-PE-Cy5-100uL
Product Name SPAG17 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
WB
Reactivity Human, Mouse, Rat
Specifications
Conjugation PE-Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SPAG17
Immunogen Range 741-840/2223
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Synonyms PF6; FLJ34497; Projection protein PF6 homolog; RP4 776P7.2; SPAG17; Sperm-associated antigen 17; SPG17_HUMAN.
Background SPAG17 (sperm associated antigen 17), also known as PF6, is a 2,223 amino acid cytoplamic protein that colocalizes with SPAG6 to microtubles. Highly expressed in testis and in organs that contain cilia-bearing cells including brain, oviduct, lung, and uterus, SPAG17 may be important for the structural integrity of the central apparatus of the sperm axoneme. SPAG17 contains two LRR (leucine-rich) repeats and may also participate in flagellar motility and male fertility.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is SPAG17 Polyclonal Antibody, PE-Cy5 Conjugated (bs-9596R-PE-Cy5) validated for, and at what dilution?

bs-9596R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SPAG17 Polyclonal Antibody, PE-Cy5 Conjugated (bs-9596R-PE-Cy5) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-9596R-PE-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

What immunogen was used to raise SPAG17 Polyclonal Antibody, PE-Cy5 Conjugated (bs-9596R-PE-Cy5)?

KLH conjugated synthetic peptide derived from human SPAG17 Immunogen range: 741-840/2223.

What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-9596R-PE-Cy5)?

PE-Cy5 on bs-9596R-PE-Cy5: excitation / emission 488nm/670nm.