Phospho-SRF (Ser77) Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9602R-BF488 |
| Product Name | Phospho-SRF (Ser77) Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human SRF around the phosphorylation site of Ser77 |
| Modification Site | Ser77 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6722 |
| Synonyms | Serum Response Factor SRF phospho S77; SRF phospho Ser77; P-SRF Ser77; c fos serum response element binding factor; Serum Response Factor SRF; c fos serum response element binding transcription factor; ELK3; ERP; MCM 1; MCM-1; MCM1; OTTHUMP00000039820; SAP2; Serum response factor; SRF; SRF serum response factor c fos serum response element binding transcription factor; SRF_HUMAN. |
| Background | This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-SRF (Ser77) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9602R-BF488) validated for, and at what dilution?
bs-9602R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-SRF (Ser77) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9602R-BF488) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig.
How should bs-9602R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-SRF (Ser77) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9602R-BF488)?
KLH conjugated synthetic phosphopeptide derived from human SRF around the phosphorylation site of Ser77
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-9602R-BF488)?
AbBy Fluor-488 on bs-9602R-BF488: excitation / emission 499nm/519nm.