ZNF828 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9607R-BF488-100uL |
| Product Name | ZNF828 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZNF828/C13orf8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | chromosome 13 open reading frame 8; zinc finger protein 828; FLJ90413; KIAA1802; Zinc finger protein KIAA1802; CHAP1_HUMAN. |
| Background | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF828, is a 812 amino acid protein that contains one C2H2-type zinc finger and is localized to the cytoplasm and the nucleus. The gene encoding ZNF828 maps to chromosome 13. Comprising nearly 4% of the human genome, chromosome 13 contains around 114 million base pairs and encodes over 400 genes. Chromosome 13 houses key tumor suppressor genes, including BRCA2 and RB1, which are associated with breast cancer susceptibility and retinoblastoma, respectively. Trisomy 13, also known as Patau syndrome, is deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is ZNF828 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9607R-BF488) validated for, and at what dilution?
bs-9607R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZNF828 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9607R-BF488) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Horse.
How should bs-9607R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZNF828 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9607R-BF488)?
KLH conjugated synthetic peptide derived from human ZNF828/C13orf8
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-9607R-BF488)?
AbBy Fluor-488 on bs-9607R-BF488: excitation / emission 499nm/519nm.