PIBF1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9612R-Cy3 |
| Product Name | PIBF1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PIBF1/C13orf24 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10464 |
| Synonyms | PIBF 1; PIBF; PIBF1; PIBF-1; Progesterone immunomodulatory binding factor 1; Progesterone induced blocking factor 1; Progesterone-Induced Blocking Factor 1; RP11 505F3.1; PIBF1_HUMAN; C13orf24. |
| Background | PIBF is synthesized during pregnancy in response to progesterone by progesterone receptor-positive T lymphocytes (mostly gamma-delta T cells). In the presence of PIBF, natural killer (NK) cells inhibit the release of perforin from storage granules and therefore fail to lyse target cells. In humans, the amount of cells that express PIBF is significantly higher in healthy pregnant women than in women at risk for premature pregnancy termination. Full-length PIBF is associated with the nucleus, whereas secretion of shorter forms is induced by activation of the cell. Research suggests that PIBF functions as a transcription factor in its full-length form, while smaller forms may act as cytokines. The PIBF gene encodes a deduced hydrophilic 757-amino acid alpha-helical protein with an N-terminal signal sequence, a leucine zipper motif, a basic zipper sequence, a PEST sequence, a nuclear localization signal, an endoplasmic reticulum membrane retention signal, and many presumeed N-glycosylation and phosphorylation sites. |
| Application Dilution | |
| IF | WB1:300-5000 |
Frequently asked questions
Which applications is PIBF1 Polyclonal Antibody, Cy3 Conjugated (bs-9612R-Cy3) validated for, and at what dilution?
bs-9612R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PIBF1 Polyclonal Antibody, Cy3 Conjugated (bs-9612R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9612R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PIBF1 Polyclonal Antibody, Cy3 Conjugated (bs-9612R-Cy3)?
KLH conjugated synthetic peptide derived from human PIBF1/C13orf24
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9612R-Cy3)?
Cy3 on bs-9612R-Cy3: excitation / emission 512,550nm/570,615nm.