Tropomyosin Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin-embedded Human Myocardial tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with Pan-Tropomyosin Polyclonal Antibody, Unconjugated (bs-9622R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.$

Paraformaldehyde-fixed, paraffin-embedded Human Myocardial tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with Pan-Tropomyosin Polyclonal Antibody, Unconjugated (bs-9622R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Sheep
Zebrafish

Overview
Catalog #	bs-9622R
Product Name	Tropomyosin Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Specificity	This antibody will detect all 4 Tropomyosin proteins: TPM1, TPM2, TPM3, and TPM4
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Sheep, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TPM1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7168
Swiss Prot	P09493
Subcellular location	Cytoskeleton
Synonyms	TPM1; Tropomyosin alpha-1 chain; Alpha-tropomyosin; Tropomyosin-1; C15orf13; TMSA; TPM2; Tropomyosin beta chain; Beta-tropomyosin; Tropomyosin-2; TMSB; TPM3; Tropomyosin alpha-3 chain; Gamma-tropomyosin; Tropomyosin-3; TPM4; Tropomyosin alpha-4 chain; TM30p1; Tropomyosin-4
Background	Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200