KLHL36 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9628R-BF488 |
| Product Name | KLHL36 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KLHL36/C16orf44 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | Kelch like 36; Kelch like protein 36; kelch-like 36 Drosophila; Kelch-like protein 36; KLH36_HUMAN; KLHL 36; KLHL-36. |
| Background | C16orf44 is a 616 amino acid protein that contains six Kelch repeats, one BTB/POZ domain and one BTB/Kelch associated (BACK) domain. C16orf44 is believed to play a role in protein ubiquitination and may function as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex. E3 ligases accept a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfer that residue to a protein that is targeted for degradation. Specifically, C16orf44 interacts with CUL-3, a member of the cullin family of mediators that participate in the selective targeting of proteins for ubiquitin-mediated proteolysis. Due to alternative splicing events, two isoforms of C16orf44 are expressed. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is KLHL36 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9628R-BF488) validated for, and at what dilution?
bs-9628R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KLHL36 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9628R-BF488) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Horse.
How should bs-9628R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KLHL36 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9628R-BF488)?
KLH conjugated synthetic peptide derived from human KLHL36/C16orf44
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-9628R-BF488)?
AbBy Fluor-488 on bs-9628R-BF488: excitation / emission 499nm/519nm.