KCTD11 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9637R-Cy5 |
| Product Name | KCTD11 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KCTD11 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 147040 |
| Synonyms | C17orf36; MGC 129844; Potassium channel tetramerisation domain containing 11; REN; Retinoic acid EGF NGF induced gene protein; C 17 orf 36; KCD11_HUMAN. |
| Background | The KCTD11 gene encodes a protein that has been identified as a suppressor of Hedgehog signaling. Its inactivation might lead to a deregulation of the tumor promoting Hedgehog pathway in medulloblastoma. Plays a role as a marker and a regulator of neuronal differentiation; Up-regulated by a variety of neurogenic signals, such as retinoic acid, epidermal growth factor/EGF and NGFB/nerve growth factor. Induces apoptosis, growth arrest and the expression of cyclin-dependent kinase inhibitor CDKN1B. Plays a role as a tumor repressor and inhibits cell growth and tumorigenicity of medulloblastoma (MDB). Functions as antagonist of the Hedgehog pathway on cell proliferation and differentiation by affecting the nuclear transfer of transcrition factor GLI1, thus maintaining cerebellar granule cells in undifferentiated state. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is KCTD11 Polyclonal Antibody, Cy5 Conjugated (bs-9637R-Cy5) validated for, and at what dilution?
bs-9637R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KCTD11 Polyclonal Antibody, Cy5 Conjugated (bs-9637R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9637R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KCTD11 Polyclonal Antibody, Cy5 Conjugated (bs-9637R-Cy5)?
KLH conjugated synthetic peptide derived from human KCTD11
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9637R-Cy5)?
Cy5 on bs-9637R-Cy5: excitation / emission 625,650nm/670nm.