C18orf1 Polyclonal Antibody, PE-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9652R-PE-Cy7-100uL |
| Product Name | C18orf1 Polyclonal Antibody, PE-Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Sheep |
| Specifications | |
| Conjugation | PE-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human C18orf1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 753 |
| Swiss Prot | O15165 |
| Subcellular location | Lumenal |
| Synonyms | C18orf1; Low-density lipoprotein receptor class A domain-containing protein 4; LDLRAD4 |
| Background | Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is C18orf1 Polyclonal Antibody, PE-Cy7 Conjugated (bs-9652R-PE-Cy7) validated for, and at what dilution?
bs-9652R-PE-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does C18orf1 Polyclonal Antibody, PE-Cy7 Conjugated (bs-9652R-PE-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Sheep.
How should bs-9652R-PE-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise C18orf1 Polyclonal Antibody, PE-Cy7 Conjugated (bs-9652R-PE-Cy7)?
KLH conjugated synthetic peptide derived from human C18orf1
What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bs-9652R-PE-Cy7)?
PE-Cy7 on bs-9652R-PE-Cy7: excitation / emission 488nm/780nm.