E3 ubiquitin ligase Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-9663R-Cy7
Product Name E3 ubiquitin ligase Polyclonal Antibody, Cy7 Conjugated
Applications WB, IF(IHC-P)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human E3 ubiquitin ligase/RNF218
Immunogen Range 51-150/352
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 79594
Swiss Prot Q969V5
Subcellular location Mitochondrion
Synonyms GIDE; MAPL; MULAN; RNF218; C1orf166; Mitochondrial ubiquitin ligase activator of NFKB 1; E3 SUMO-protein ligase MUL1; E3 ubiquitin-protein ligase MUL1; Growth inhibition and death E3 ligase; Mitochondrial-anchored protein ligase; Putative NF-kappa-B-activating protein 266; RING finger protein 218; MUL1
Background Exhibits weak E3 ubiquitin-protein ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Can ubiquitinate AKT1 preferentially at 'Lys-284' involving 'Lys-48'-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteosomal degradation. Proposed to preferentially act as a SUMO E3 ligase at physiological concentrations. Plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. The function may implicate its abilty to sumoylate DNM1L. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. Involved in the modulation of innate immune defense against viruses by inhibiting DDX58-dependent antiviral response. Can mediate DDX58 sumoylation and disrupt its polyubiquitination.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200