ZNF144 Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9673R-Biotin-100uL |
| Product Name | ZNF144 Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MEL18 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 7703 |
| Swiss Prot | P35227 |
| Subcellular location | Nucleus |
| Synonyms | MEL-18; RNF110; ZNF144; Polycomb group RING finger protein 2; DNA-binding protein Mel-18; RING finger protein 110; Zinc finger protein 144; PCGF2; MEL18 |
| Background | Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Is not functionally redundant with BMI1; unlike BMI1 does not stimulate the E3 ubiquitin-protein ligase activity in a reconstituted PRC1-like complex (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | |
Frequently asked questions
Which applications is ZNF144 Polyclonal Antibody, Biotin Conjugated (bs-9673R-Biotin) validated for, and at what dilution?
bs-9673R-Biotin is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZNF144 Polyclonal Antibody, Biotin Conjugated (bs-9673R-Biotin) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9673R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZNF144 Polyclonal Antibody, Biotin Conjugated (bs-9673R-Biotin)?
KLH conjugated synthetic peptide derived from human MEL18